Camel Naïve VHH Library as phage particles: (5×10^13pfu, Ampicillin resistant), supplied in 50×100μL PBS with 15% glycerol ;
E.coli TG1 host strain: K12 D(lac-pro), supE, thi, hsdD5/F’[traD36, proAB, lacIq, lacZDM15] ;
M13K07 helper phage: 5.0×10^12 pfu/mL supplied in 1000μL PBS with 15% glycerol ;
Sequencing primer p-F1 : 5'-TGTGGAATTGTGAGCGGATAAC-3' ;
Phage Display Screening Protocols:《Selection in Microporous plate》、《Selection on Magnetic beads》、《Selection on Magnetic cells》.