Camel Naïve VHH Library as phage particles: (5×10^12 pfu, Ampicillin resistant), supplied in 5×100μL PBS with 15% glycerol.

E.coli TG1 host strain: K12 D(lac-pro), supE, thi, hsdD5/F’[traD36, proAB, lacIq, lacZDM15].  

M13K07 helper phage: 5.0×10^12 pfu/mL supplied in 1000μL PBS with 15% glycerol

Sequencing primer p-F1 : 5'-TGTGGAATTGTGAGCGGATAAC-3' 

Phage Display Screening Protocols：《Selection in Microporous plate》、《Selection on Magnetic beads》、《Selection on Magnetic cells》