Donors : 1000
Naïve Human scFv Library Capacity: >1.0×10^12 transformants ;
Naïve Human scFv Library as phage particles: 5×10^12 pfu, supplied in 500uL PBS with 15% glycerol ;
E.coli TG1 host strain: K12 D(lac-pro), supE, thi, hsdD5/F’[traD36, proAB, lacIq, lacZDM15] ;
M13KO 7 helper phage: 5.0×10^12 pfu/mL supplied in 1000μL PBS with 15% glycerol ;
Sequencing primer L primer: 5'-TGGAATTGTGAGCGGATAACAATT-3' ;
Sequencing primer S primer: 5'-GTAAATGAATTTTCTGTATGAGG-3' .